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Biophys J. 2011 Dec 7;101(11):L57-9. doi: 10.1016/j.bpj.2011.10.028.

Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen.

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1
Biology Department, The Johns Hopkins University, Baltimore, Maryland, USA.

Abstract

Changes in the clustering of surface receptors modulate cell responses to ligands. Hence, global measures of receptor clustering can be useful for characterizing cell states. Using T cell receptor for antigen as an example, we show that k-space image correlation spectroscopy of quantum dots blinking detects T cell receptor clusters on a scale of tens of nanometers and reports changes in clustering after T cell activation. Our results offer a general approach to the global analysis of lateral organization and receptor clustering in single cells, and can thus be applied when the cell type of interest is rare.

PMID:
22261075
PMCID:
PMC3297813
DOI:
10.1016/j.bpj.2011.10.028
[Indexed for MEDLINE]
Free PMC Article
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