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Cell Reprogram. 2012 Feb;14(1):1-7. doi: 10.1089/cell.2011.0048. Epub 2012 Jan 18.

Established preblastocyst- and blastocyst-derived ES cell lines have highly similar gene expression profiles, despite their differing requirements for derivation culture conditions.

Author information

1
Center for Regenerative Biology, University of Connecticut, Storrs, Connecticut 06269-4243, USA. chulkim@uchicago.edu

Abstract

The efficiency of embryonic stem (ES) cell derivation relies on an optimized culture medium and techniques for treating preimplantation stage embryos. Recently, ES cell derivation from the preblastocyst developmental stage was reported by removing the zona pellucida from embryos of the most efficient strain for ES cell derivation (129Sv) during early preimplantation. Here, we showed that ES cells can be efficiently derived and maintained in a modified medium (MEMα), from preblastocysts of a low-efficiency mouse strain (a hybrid consisting of 50% B6, 25% CBA, and 25% DBA). Preblastocyst-derived ES cell lines were normal in terms of pluripotency-related protein expression, and chromosome number. Also, preblastocyst-derived ES cell lines from various culture conditions showed pluripotency in vivo through teratoma analysis. Interestingly, ES cell lines produced from preblastocysts and blastocysts, regardless of the derivation culture conditions, are nearly indistinguishable by their global gene expression profiles.

PMID:
22257162
PMCID:
PMC3275092
DOI:
10.1089/cell.2011.0048
[Indexed for MEDLINE]
Free PMC Article

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