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Antimicrob Agents Chemother. 2012 Apr;56(4):1774-83. doi: 10.1128/AAC.05395-11. Epub 2012 Jan 17.

Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors.

Author information

1
Department of Chemistry and A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.

Abstract

A reporter construct was created on the basis of the transcription attenuator region of the Escherichia coli tryptophan operon. Dual-fluorescent-protein genes for red fluorescent protein and cerulean fluorescent protein were used as a sensor and internal control of gene expression. The sequence of the attenuator was modified to avoid tryptophan sensitivity while preserving sensitivity to ribosome stalling. Antimicrobial compounds which cause translation arrest at the stage of elongation induce the reporter both in liquid culture and on an agar plate. This reporter could be used for high-throughput screening of translation inhibitors.

PMID:
22252829
PMCID:
PMC3318315
DOI:
10.1128/AAC.05395-11
[Indexed for MEDLINE]
Free PMC Article

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