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Sensors (Basel). 2011;11(12):11335-42. doi: 10.3390/s111211335. Epub 2011 Nov 28.

Time-resolved fluorescence immunoassay for C-reactive protein using colloidal semiconducting nanoparticles.

Author information

1
Laboratory of Biophysics, University of Turku, 20520 Turku, Finland. harri.harma@utu.fi

Abstract

Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.

KEYWORDS:

bioaffinity assays; colloidal nanoparticles; quantum dots; sensing; time-gated fluorescence

PMID:
22247668
PMCID:
PMC3251985
DOI:
10.3390/s111211335
[Indexed for MEDLINE]
Free PMC Article

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