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Nat Methods. 2012 Jan 15;9(3):255-8. doi: 10.1038/nmeth.1854.

Serial two-photon tomography for automated ex vivo mouse brain imaging.

Author information

1
TissueVision, Inc., Cambridge, Massachusetts, USA.

Abstract

Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

PMID:
22245809
PMCID:
PMC3297424
DOI:
10.1038/nmeth.1854
[Indexed for MEDLINE]
Free PMC Article

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