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Br J Cancer. 2012 Feb 14;106(4):727-32. doi: 10.1038/bjc.2011.603. Epub 2012 Jan 12.

FGFR2 gene amplification and clinicopathological features in gastric cancer.

Author information

1
Department of Genome Biology, Kinki University Faculty of Medicine, Osaka 589-8511, Japan.

Abstract

BACKGROUND:

Frequency of FGFR2 amplification, its clinicopathological features, and the results of high-throughput screening assays in a large cohort of gastric clinical samples remain largely unclear.

METHODS:

Drug sensitivity to a fibroblast growth factor receptor (FGFR) inhibitor was evaluated in vitro. The gene amplification of the FGFRs in formalin-fixed, paraffin-embedded (FFPE) gastric cancer tissues was determined by a real-time PCR-based copy number assay and fluorescence in situ hybridisation (FISH).

RESULTS:

FGFR2 amplification confers hypersensitivity to FGFR inhibitor in gastric cancer cell lines. The copy number assay revealed that 4.1% (11 out of 267) of the gastric cancers harboured FGFR2 amplification. No amplification of the three other family members (FGFR1, 3 and 4) was detected. A FISH analysis was performed on 7 cases among 11 FGFR2-amplified cases and showed that 6 of these 7 cases were highly amplified, while the remaining 1 had a relatively low grade of amplification. Although the difference was not significant, patients with FGFR2 amplification tended to exhibit a shorter overall survival period.

CONCLUSION:

FGFR2 amplification was observed in 4.1% of gastric cancers and our established PCR-based copy number assay could be a powerful tool for detecting FGFR2 amplification using FFPE samples. Our results strongly encourage the development of FGFR-targeted therapy for gastric cancers with FGFR2 amplification.

PMID:
22240789
PMCID:
PMC3322955
DOI:
10.1038/bjc.2011.603
[Indexed for MEDLINE]
Free PMC Article

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