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Biochim Biophys Acta. 1990 Nov 9;1036(2):138-42.

Identification of non-equilibrium glycolytic reactions in suspension-cultured plant cells.

Author information

1
Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan.

Abstract

Levels of all enzymes and metabolites involved in glycolysis were determined in suspension-cultured Catharanthus roseus cells. From both the maximum catalytic activities of the enzymes and comparisons of mass-action ratios with the apparent equilibrium constants for the reactions, it is concluded that the reactions catalysed by hexokinase, fructokinase, phosphofructokinase and pyruvate kinase are far from equilibrium, whereas the other reactions including that catalysed by pyrophosphate-fructose-6-phosphate 1-phosphotransferase, are close to equilibrium in vivo.

PMID:
2223831
DOI:
10.1016/0304-4165(90)90025-r
[Indexed for MEDLINE]

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