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Methods Mol Biol. 2012;840:155-70. doi: 10.1007/978-1-61779-516-9_19.

Generating barcoded libraries for multiplex high-throughput sequencing.

Author information

1
Allan Wilson Centre for Molecular Ecology and Evolution, Department of Anatomy and Structural Biology, University of Otago, Dunedin 9016, New Zealand. michael.knapp@otago.ac.nz

Abstract

Molecular barcoding is an essential tool to use the high throughput of next generation sequencing platforms optimally in studies involving more than one sample. Various barcoding strategies allow for the incorporation of short recognition sequences (barcodes) into sequencing libraries, either by ligation or polymerase chain reaction (PCR). Here, we present two approaches optimized for generating barcoded sequencing libraries from low copy number extracts and amplification products typical of ancient DNA studies.

PMID:
22237533
DOI:
10.1007/978-1-61779-516-9_19
[Indexed for MEDLINE]

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