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Lab Anim Res. 2011 Dec;27(4):339-42. doi: 10.5625/lar.2011.27.4.339. Epub 2011 Dec 19.

Subcellular localization of the transmembrane inner ear (Tmie) protein in a stable Tmie-expressing cell line.

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1
Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Korea.

Abstract

Mutations in the transmembrane inner ear (Tmie) gene, which encodes the Tmie protein, have been attributed to deafness autosomal recessive 6 (DFNB6), an autosomal nonsyndromic recessive hearing loss disorder. Although the Tmie gene was identified a few years ago, little is known about subcellular localization of the Tmie protein. In order to address this, we developed a stable cell line expressing Tmie protein. The expression of Myc-tagged Tmie protein was confirmed by Western blot analysis using an anti-Myc antibody and localization of the Tmie protein was confirmed by immunostaining, using the anti-Myc antibody as well as the anti-tmie antibody. Our study demonstrates that the Tmie protein is localized mostly in the cellular membrane and to a lesser extent in cytoplasm. These results suggest that our Tmie expressing stable cell line provides a suitable in vitro model to explore Tmie synthesis and functions.

KEYWORDS:

HEK293; Transmembrane inner ear protein; hearing loss disorder; subcellular localization

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