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J Clin Virol. 2012 Mar;53(3):262-4. doi: 10.1016/j.jcv.2011.12.007. Epub 2012 Jan 9.

Evaluation of the RIDAQuick norovirus immunochromatographic test kit.

Author information

1
Laboratory of Viral Diseases, Wadsworth Center, Albany, NY, USA. battagli@wadsworth.org

Abstract

BACKGROUND:

Norovirus infections occur frequently and are widespread throughout the US population causing greater than half of all foodborne gastroenteritis cases. A rapid norovirus assay would be a useful clinical tool for identification of this common virus in gastroenteritis patient samples, thereby identifying outbreaks and facilitating rapid implementation of control measures.

OBJECTIVES:

To determine the suitability of the RIDAQuick norovirus kit as a clinical tool by determining the specificity and sensitivity of the assay, and its cross-reactivity with other enteric viruses.

STUDY DESIGN:

Archived stool specimens containing norovirus genogroup I or II or other viruses were tested using the RIDAQuick norovirus assay and results compared to those obtained with real-time RT-PCR.

RESULTS:

We tested 62 samples: 19 norovirus genogroup I, 25 genogroup II samples, and 18 norovirus negative samples. Compared to PCR results, RIDAQuick assay sensitivity was 61.4%, and specificity was 100%. The low sensitivity was mainly due to poor results with genogroup I specimens; only 11 of 19 were detected. Additionally, samples of four other common enteric viruses all tested negative with the RIDAQuick assay.

CONCLUSIONS:

The RIDAQuick kit effectively detects norovirus genogroup II strains, but not genogroup I strains. We found no cross-reactivity with several common enteric viruses. As most norovirus cases are currently genogroup II strains, positive results with RIDAQuick can be used for rapid detection of norovirus in a large percentage of cases, thus also aiding in identification of outbreaks. However, final confirmation and negative results require further testing with more sensitive methods.

PMID:
22226980
DOI:
10.1016/j.jcv.2011.12.007
[Indexed for MEDLINE]
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