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Biochem Biophys Res Commun. 2012 Jan 20;417(3):996-1001. doi: 10.1016/j.bbrc.2011.12.069. Epub 2011 Dec 27.

Coordinated regulation of IL-4 and IL-13 expression in human T cells: 3C analysis for DNA looping.

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Department of Respiratory Disease, The First Affiliated Hospital of Nanjing Medical University, China.


Asthma is a chronic allergic disorder characterised by chronic inflammation. The balance of type I and type II (CD4+) T helper cells is of critical importance. In asthma there is an overexpression of T(H)2 cytokines, such as IL-4, IL-5 and IL-13. The genes encoding these cytokines are located together the same chromosomal region, 5q31.1 in humans. Here we confirm a central role for the transcription factors NFAT and GATA3 in the regulation of human IL-4 and IL-13. Chromatin Conformation Capture (3C) demonstrated the formation of specific ligation products containing spliced IL-4 and IL-13 DNA sequences in human T(H)2 polarised HuT-78 cells. This suggests that co-ordinate expression of T(H)2 cytokines, under the control of GATA3 and NFAT1 is due to the formation of a chromatin hub by DNA looping.

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