Identification of two novel HLA-A*02 variants, A*02:319 and A*02:01:64, in two Taiwanese marrow stem cell donors by sequence-based typing

K L Yang; S K Lee; S Y Yang; R H Kao; C L Lin; P Y Lin

doi:10.1111/j.1744-313X.2011.01080.x

Identification of two novel HLA-A02 variants, A02:319 and A*02:01:64, in two Taiwanese marrow stem cell donors by sequence-based typing

Int J Immunogenet. 2012 Jun;39(3):261-3. doi: 10.1111/j.1744-313X.2011.01080.x. Epub 2012 Jan 5.

Authors

K L Yang¹, S K Lee, S Y Yang, R H Kao, C L Lin, P Y Lin

Affiliation

¹ Laboratory of Immunogenetics and Cord Blood Bank, Buddhist Tzu Chi Stem Cells Centre and Marrow Donor Registry, Buddhist Tzu Chi General Hospital, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw

PMID: 22221707
DOI: 10.1111/j.1744-313X.2011.01080.x

Abstract

We report here two novel variants of HLA-A*02 allele, A*02:319 and A*02:01:64, discovered in two Taiwanese unrelated volunteer bone marrow donors by sequence-based typing (SBT) method. The DNA sequence of A*02:319 is identical to A*02:07 in exons 2 and 3 but varies with one nucleotide at codon 9 (TTC->TCC). The variation caused one amino acid substitution at residue 9 (F->S). On the other hand, the DNA sequence of A*02:01:64 is identical to the sequence of A*02:01:01:01 in exons 2 and 3 except a silent mutation at codon 114 (CAC->CAT). The probable HLA-A, HLA-B and HLA-DRB1 haplotypes in association with A*02:319 and A*02:01:64 were deduced as A*02:319-B*46:01-DRB1*04 and A*02:01:64-B*38:02-DRB1*16:02, respectively.

MeSH terms

Alleles
Asian People / genetics
Base Sequence
Blood Donors*
Bone Marrow Cells / cytology
Genetic Variation*
Genotyping Techniques / methods*
HLA-A2 Antigen / genetics*
Haplotypes
Hematopoietic Stem Cells / cytology
Humans
Polymorphism, Genetic
Taiwan

Substances

HLA-A*02 antigen
HLA-A2 Antigen