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PLoS One. 2011;6(12):e28879. doi: 10.1371/journal.pone.0028879. Epub 2011 Dec 21.

A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing.

Author information

1
Department of Veterinary Medicine, The University of Cambridge, Cambridge, United Kingdom.

Abstract

Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.

PMID:
22216131
PMCID:
PMC3244418
DOI:
10.1371/journal.pone.0028879
[Indexed for MEDLINE]
Free PMC Article

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