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J Mol Recognit. 2012 Jan;25(1):11-4. doi: 10.1002/jmr.1164.

The macromolecular state of A-kinase anchoring protein.

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Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2.


The amendment of the interpretation of recently published size-exclusion chromatography (SEC) data for A-kinase anchoring protein (AKAP12) on Sephacryl-S400 has led to an increase in the estimated size of the supermolecular state from 840 to at least 6000‚ÄČkDa. Although size-exclusion chromatography has sufficed to demonstrate unequivocally the existence of this 190-kDa scaffold protein in a supermolecular state, any quantitative estimate of the oligomer stoichiometry is shown to be precluded by failure of this empirical procedure to incorporate allowance for any deviation from globular shape--an important consideration in view of the extended structures exhibited by other extracellular matrix proteins.

[Indexed for MEDLINE]

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