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J Clin Microbiol. 2012 Mar;50(3):1122-4. doi: 10.1128/JCM.05121-11. Epub 2012 Jan 11.

Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia.

Author information

1
Johns Hopkins University, Baltimore, Maryland USA. kyj2@duke.edu

Abstract

Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

PMID:
22205823
PMCID:
PMC3295147
DOI:
10.1128/JCM.05121-11
[Indexed for MEDLINE]
Free PMC Article

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