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Clin Vaccine Immunol. 2012 Feb;19(2):288-92. doi: 10.1128/CVI.05583-11. Epub 2011 Dec 28.

Human endogenous retrovirus K(HML-2) Gag- and Env-specific T-cell responses are infrequently detected in HIV-1-infected subjects using standard peptide matrix-based screening.

Author information

1
Department of Immunology, University of Toronto, Toronto, Ontario, Canada. brad.jones@utoronto.ca

Abstract

T-cell responses to human endogenous retrovirus (HERV) K(HML-2) Gag and Env were mapped in HIV-1-infected subjects using 15 mer peptides. Small peptide pools and high concentrations were used to maximize sensitivity. In the 23 subjects studied, only three bona fide HERV-K(HML-2)-specific responses were detected. At these high peptide concentrations, we detected false-positive responses, three of which were mapped to an HIV-1 Gag peptide contaminant. Thus, HERV-K(HML-2) Gag- and Env-specific T-cell responses are infrequently detected by 15 mer peptide mapping.

PMID:
22205657
PMCID:
PMC3272926
DOI:
10.1128/CVI.05583-11
[Indexed for MEDLINE]
Free PMC Article

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