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Arterioscler Thromb Vasc Biol. 2012 Mar;32(3):662-8. doi: 10.1161/ATVBAHA.111.242685. Epub 2011 Dec 22.

Matrix metalloproteinase-2 proteolysis of calponin-1 contributes to vascular hypocontractility in endotoxemic rats.

Author information

1
Department of Pharmacology, Cardiovascular Research Centre, Mazankowski Alberta Heart Institute, 4-62 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada.

Abstract

OBJECTIVE:

Matrix metalloproteinase (MMP)-2 is activated in aorta during endotoxemia and plays a role in the hypocontractility to vasoconstrictors. Calponin-1 is a regulator of vascular smooth muscle tone with similarities to troponin, a cardiac myocyte protein that is cleaved by MMP-2 in myocardial oxidative stress injuries. We hypothesized that calponin-1 may be proteolyzed by MMP-2 in endotoxemia-induced vascular hypocontractility.

METHODS AND RESULTS:

Rats were given a nonlethal dose of bacterial lipopolysaccharide (LPS) or vehicle. Some rats were given the MMP inhibitors ONO-4817 or doxycycline. Six hours later, plasma nitrate+nitrite increased >15-fold in LPS-treated rats, an effect unchanged by doxycycline. Both ONO-4817 and doxycycline prevented LPS-induced aortic hypocontractility to phenylephrine. LPS activated MMP-2 in the aorta by S-glutathiolation. Calponin-1 levels decreased by 25% in endotoxemic aortae, which was prevented by doxycycline. Calponin-1 and MMP-2 coimmunoprecipitated and both exhibited uniform cytosolic staining in medial vascular smooth muscle cells. In vitro incubation of calponin-1 with MMP-2 led to calponin-1 degradation and appearance of its cleavage product.

CONCLUSION:

Calponin-1 is a target of MMP-2, which contributes to endotoxemia-induced vascular hypocontractility.

PMID:
22199370
DOI:
10.1161/ATVBAHA.111.242685
[Indexed for MEDLINE]

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