Format

Send to

Choose Destination
J Exp Bot. 2012 Feb;63(4):1725-33. doi: 10.1093/jxb/err403. Epub 2011 Dec 22.

Cross-talk between calcium signalling and protein phosphorylation at the thylakoid.

Author information

1
Department of Biochemistry and Cell Biology, MFPL, University of Vienna, Dr. Bohrgasse 9, A-1030, Vienna, Austria.

Abstract

The role of protein phosphorylation for adjusting chloroplast functions to changing environmental needs is well established, whereas calcium signalling in the chloroplast is only recently becoming appreciated. The work presented here explores the potential cross-talk between calcium signalling and protein phosphorylation in chloroplasts and provides the first evidence for targets of calcium-dependent protein phosphorylation at the thylakoid membrane. Thylakoid proteins were screened for calcium-dependent phosphorylation by 2D gel electrophoresis combined with phospho-specific labelling and PsaN, CAS, and VAR1, among other proteins, were identified repeatedly by mass spectrometry. Subsequently their calcium-dependent phosphorylation was confirmed in kinase assays using the purified proteins and chloroplast extracts. This is the first report on the protein targets of calcium-dependent phosphorylation of thylakoid proteins and provides ground for further studies in this direction.

PMID:
22197893
PMCID:
PMC3970089
DOI:
10.1093/jxb/err403
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center