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Virology. 1990 Oct;178(2):603-5.

Vaccinia virus gene D7R encodes a 20,000-dalton subunit of the viral DNA-dependent RNA polymerase.

Author information

1
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907.

Abstract

The polypeptide encoded by the vaccinia virus open reading frame D7R was synthesized in bacteria. Immunization of rabbits with the polypeptide resulted in antibodies that specifically recognized a virion polypeptide of 20,000 daltons. The immunoreactivity with the 20,000-dalton polypeptide was found to coincide with the virion-associated DNA-dependent RNA polymerase through DEAE-cellulose chromatography and glycerol gradient sedimentation. These results argue that the product of the vaccinia open reading frame D7R is a subunit of the viral RNA polymerase.

PMID:
2219712
DOI:
10.1016/0042-6822(90)90362-u
[Indexed for MEDLINE]

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