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Appl Environ Microbiol. 2012 Mar;78(5):1584-8. doi: 10.1128/AEM.05937-11. Epub 2011 Dec 22.

Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay.

Author information

1
Department of Environmental Sciences, University of California, Riverside, CA, USA .

Abstract

A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection.

PMID:
22194298
PMCID:
PMC3294457
DOI:
10.1128/AEM.05937-11
[Indexed for MEDLINE]
Free PMC Article

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