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Cold Spring Harb Protoc. 2012 Jan 1;2012(1):124-5. doi: 10.1101/pdb.prot067553.

Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks.

Abstract

Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production and harvesting of vector from cells grown in T75 tissue-culture flasks. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.

PMID:
22194266
DOI:
10.1101/pdb.prot067553
[Indexed for MEDLINE]

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