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Biotechnol Biofuels. 2011 Dec 22;4(1):61. doi: 10.1186/1754-6834-4-61.

Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.

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1
Centre of Marine Sciences, University of Algarve, Faro, Portugal. jvarela@ualg.pt.

Abstract

We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the growth of the same cultures in liquid medium. This approach enabled us to isolate a biodiverse collection of several axenic and unialgal cultures of different phyla.

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