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J Biomed Opt. 2011 Dec;16(12):126012. doi: 10.1117/1.3662457.

Myofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy.

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1
Clemson University, Department of Bioengineering and COMSET, Clemson, South Carolina 29634, USA.

Abstract

We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubator is an effective tool for investigation of dynamic myofibrillogenesis.

PMID:
22191929
PMCID:
PMC3245745
DOI:
10.1117/1.3662457
[Indexed for MEDLINE]
Free PMC Article
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