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Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):511-5. doi: 10.1016/j.vetimm.2011.11.017. Epub 2011 Nov 25.

Characterization of an anti-rainbow trout (Oncorhynchus mykiss) CD3ε monoclonal antibody.

Author information

1
Department of Biology, University of Massachusetts Dartmouth, 285 Old Westport Road, Dartmouth, MA 02747, USA.

Abstract

This study characterizes a monoclonal antibody (mAb) produced against the cytoplasmic tail region of the epsilon chain of the CD3 (CD3ε) transmembrane protein found on T lymphocytes of rainbow trout (Oncorhynchus mykiss). Flow cytometry and fluorescent microscopy conducted on trout leukocytes with the anti-trout CD3ε mAb showed a distinctive population of IgM(-) CD3e(+) lymphocytes fitting the expected profile of T-cells. Immunoprecipitation of lysates derived from trout lymphocytes revealed a 19 kDa protein and peptide analysis confirmed its specificity for CD3ɛ. In vitro proliferation assays with T-cell mitogens, ConA and PHA, resulted in a 3 fold increase in the percentage of CD3ɛ+ lymphocytes compared to LPS and control cultures. The mAb characterized in this study will be useful in further elucidation for both the role and distribution of T lymphocytes in the teleost immune system.

PMID:
22188783
DOI:
10.1016/j.vetimm.2011.11.017
[Indexed for MEDLINE]

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