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Mol Biosyst. 2012 Feb;8(2):491-4. doi: 10.1039/c2mb05422a. Epub 2011 Dec 19.

Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole.

Author information

1
School of Materials Science, Japan Advanced institute of Science and Technology, Nomi, Ishikawa, Japan. kenzo@jaist.ac.jp

Abstract

To develop a covalent and specific labeling method for single- and double-stranded plasmid DNA, photoresponsive oligonucleotide containing 3-cyanovinylcarbazole nucleoside was adopted. Single- and double-stranded plasmid DNA was successfully labeled/de-labeled with Cy3 and/or biotin by photoirradiation.

PMID:
22183016
DOI:
10.1039/c2mb05422a
[Indexed for MEDLINE]

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