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Reprod Toxicol. 2012 Apr;33(2):174-87. doi: 10.1016/j.reprotox.2011.10.018. Epub 2011 Dec 9.

Zebrafish developmental screening of the ToxCast™ Phase I chemical library.

Author information

1
National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC 27712, USA. Padilla.Stephanie@epa.gov

Abstract

Zebrafish (Danio rerio) is an emerging toxicity screening model for both human health and ecology. As part of the Computational Toxicology Research Program of the U.S. EPA, the toxicity of the 309 ToxCast™ Phase I chemicals was assessed using a zebrafish screen for developmental toxicity. All exposures were by immersion from 6-8 h post fertilization (hpf) to 5 days post fertilization (dpf); nominal concentration range of 1 nM-80 μM. On 6 dpf larvae were assessed for death and overt structural defects. Results revealed that the majority (62%) of chemicals were toxic to the developing zebrafish; both toxicity incidence and potency was correlated with chemical class and hydrophobicity (logP); and inter-and intra-plate replicates showed good agreement. The zebrafish embryo screen, by providing an integrated model of the developing vertebrate, compliments the ToxCast assay portfolio and has the potential to provide information relative to overt and organismal toxicity.

PMID:
22182468
DOI:
10.1016/j.reprotox.2011.10.018
[Indexed for MEDLINE]

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