Format

Send to

Choose Destination
See comment in PubMed Commons below
J Immunoassay Immunochem. 2012 Jan;33(1):48-58. doi: 10.1080/15321819.2011.591479.

Investigating membrane morphology and quantity of immobilized protein for the development of lateral flow immunoassay.

Author information

1
School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, Penang, Malaysia.

Abstract

This study was aimed at gaining a quantitative understanding of the effect of protein quantity and membrane pore structure on protein immobilization. The concentration of immobilized protein was measured by staining with Ponceau S and measuring its color intensity. In this study, both membrane morphology and the quantity of deposited protein significantly influenced the quantity of protein immobilization on the membrane surface. The sharpness and intensity of the red protein spots varied depending on the membrane pore structure, indicating a dependence of protein immobilization on this factor. Membranes with smaller pores resulted in a higher color density, corresponding to enhanced protein immobilization and an increased assay sensitivity level. An increased of immobilized volume has a significant jagged outline on the protein spot but, conversely, no difference in binding capacity.

PMID:
22181820
DOI:
10.1080/15321819.2011.591479
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center