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J Nephrol. 2012 Sep-Oct;25(5):738-43. doi: 10.5301/jn.5000053.

C57BL/6 and 129/Sv mice: genetic difference to renal ischemia-reperfusion.

Author information

1
Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College of HUST, Wuhan, PR China.

Abstract

BACKGROUND:

C57BL/6 and 129/Sv are the 2 most commonly used strains of mice in renal ischemia-reperfusion injury (IRI) studies, yet there are currently no studies that contrast differences in the degree of renal injury after ischemia-reperfusion.

METHODS:

To evaluate renal IRI in male C57BL/6 and 129/Sv mice, we performed unilateral clamping of the left renal pedicle for 45 minutes and compared the degree of renal tissue damage and function. To measure function and tissue damage we examined: glomerular filtration rate (GFR; by inulin clearance), renal blood flow (RBF; by p-aminohippurate [PAH] clearance), renal morphology, immunohistochemistry for infiltrating leukocytes, and fibrogenic markers by Sirius red staining.

RESULTS:

After unilateral IRI, 129/sv mice had significantly less GFR and RBF disfunction at both day 14 (d14) and d28. 129/sv mice also had significantly less acute tubular necrosis on d1 and fewer infiltrating leukocytes on d28, as well as less collagen deposition on d28 than C57BL/6 mice.

CONCLUSIONS:

C57BL/6 mice were much more sensitive to damage caused by renal IRI than are 129/Sv mice.

PMID:
22180224
DOI:
10.5301/jn.5000053
[Indexed for MEDLINE]

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