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Electrophoresis. 2011 Dec;32(24):3456-66. doi: 10.1002/elps.201100247.

Recent advances in hydrophilic interaction liquid chromatography (HILIC) for structural glycomics.

Author information

1
Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.

Abstract

This review presents recent progress in employing hydrophilic interaction liquid chromatography (HILIC) for glycan and glycopeptides analysis. After an introduction of this technique, the following themes are addressed: (i) implementation of HILIC in large-scale studies for analyzing the human plasma N-glycome; (ii) the use of HILIC UPLC (ultrahigh pressure liquid chromatography) for fast high-resolution runs and its successful application with online MS for glycan and glycopeptide analysis; (iii) high-throughput profiling using HILIC solid-phase extraction in combination with MS detection; (iv) HILIC sample preparation for CE and CGE; (v) the latest glycoproteomic approaches implementing HILIC separation; (vi) future perspectives of HILIC including its use in large-scale glycoproteomics studies such as the analysis of entire glycoproteomes at the glycopeptide level.

PMID:
22180202
DOI:
10.1002/elps.201100247
[Indexed for MEDLINE]

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