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Analyst. 2012 Feb 7;137(3):595-600. doi: 10.1039/c2an15780b. Epub 2011 Dec 16.

Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance.

Author information

1
Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr, West Lafayette, IN 47907, USA.

Abstract

A convenient and fast method for quantifying urea in biofluids is demonstrated using NMR analysis and the solvent water signal as a concentration reference. The urea concentration can be accurately determined with errors less than 3% between 1 mM and 50 mM, and less than 2% above 50 mM in urine and serum. The method is promising for various applications with advantages of simplicity, high accuracy, and fast non-destructive detection. With an ability to measure other metabolites simultaneously, this NMR method is also likely to find applications in metabolic profiling and system biology.

PMID:
22179722
PMCID:
PMC4758351
DOI:
10.1039/c2an15780b
[Indexed for MEDLINE]
Free PMC Article

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