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Biophys Chem. 2012 Feb;161:50-3. doi: 10.1016/j.bpc.2011.11.003. Epub 2011 Nov 25.

Ligand-induced clustering of EGF receptors: a quantitative study by fluorescence image moment analysis.

Author information

1
Department of Physics, McGill University, Montréal, Québec, Canada.

Abstract

Fluorescence microscopy is widely used in the life sciences, but largely for qualitative imaging. Here we apply a bioanalytical technique, fluorescence image moment analysis, to demonstrate how the distribution of the fluorescent molecules can be measured directly from confocal microscopy images. We measured the oligomerization state of EGF-eGPF receptors expressed in CHO-K1 cells in situ.

PMID:
22178063
DOI:
10.1016/j.bpc.2011.11.003
[Indexed for MEDLINE]

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