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Trends Biotechnol. 2012 Apr;30(4):216-24. doi: 10.1016/j.tibtech.2011.11.004. Epub 2011 Dec 13.

Physical analysis of virus particles using electrospray differential mobility analysis.

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Department of Chemical Engineering, University of Utah, Salt Lake City, UT 84112, USA.


This review critically examines an emerging tool to measure viral clearance from biomanufacturing streams, monitor assembly of viruses and virus-like particles, rapidly identify viruses from biological milieu, assay virus neutralization, and prepare bionanoconjugates for bacterial detection. Electrospray differential mobility analysis (ES-DMA) is a tool of choice to simultaneously determine viral size and concentration because it provides full multimodal size distributions with subnanometer precision from individual capsid proteins to intact viral particles. The review contrasts ES-DMA to similar tools and highlights expected growth areas including at-line process sensing as a process analytical technology (PAT), bioseparating as a distinct unit operation, monitoring viral reactions, and interrogating virus-host protein interactions.

[Indexed for MEDLINE]

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