Format

Send to

Choose Destination
Methods Mol Biol. 2012;831:55-69. doi: 10.1007/978-1-61779-480-3_4.

Isotope labeling in mammalian cells.

Author information

1
Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Abstract

Isotope labeling of proteins represents an important and often required tool for the application of nuclear magnetic resonance (NMR) spectroscopy to investigate the structure and dynamics of proteins. Mammalian expression systems have conventionally been considered to be too weak and inefficient for protein expression. However, recent advances have significantly improved the expression levels of these systems. Here, we provide an overview of some of the recent developments in expression strategies for mammalian expression systems in view of NMR investigations.

PMID:
22167668
PMCID:
PMC3263381
DOI:
10.1007/978-1-61779-480-3_4
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center