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Methods Mol Biol. 2012;831:37-54. doi: 10.1007/978-1-61779-480-3_3.

Isotope labeling in insect cells.

Author information

1
Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance, Johann Wolfgang Goethe-University Frankfurt, Frankfurt am Main, Germany.

Abstract

Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.

PMID:
22167667
PMCID:
PMC3249615
DOI:
10.1007/978-1-61779-480-3_3
[Indexed for MEDLINE]
Free PMC Article

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