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Nucleic Acids Res. 1990 Oct 11;18(19):5673-6.

Rat DNA polymerase beta gene can join in excision repair of Escherichia coli.

Author information

1
Department of Biology, Nara Medical University, Japan.

Abstract

Though DNA polymerase I (poll) of Escherichia (E.) coli is understood to play a role in repair synthesis of excision repair, it is still obscure whether DNA polymerase beta (pol beta) plays a similar role in eukaryotic cells. To estimate the role of pol beta in excision repair processes, we inserted the rat pol beta gene into several mutant E. coli defective in a diverse set of enzymatic activities of poll. UV resistance was seen only when the 5'----3' exonuclease (exo) activity of poll molecules remained. Therefore it is suggested that 5'----3' exo activity as well as pol beta activity are essential for repair synthesis of excision repair in eukaryotic cells.

PMID:
2216761
PMCID:
PMC332299
DOI:
10.1093/nar/18.19.5673
[Indexed for MEDLINE]
Free PMC Article

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