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Cardiovasc Res. 2012 Mar 15;93(4):674-81. doi: 10.1093/cvr/cvr330. Epub 2011 Dec 13.

Pregnane X receptor regulates drug metabolism and transport in the vasculature and protects from oxidative stress.

Author information

1
Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK. k.swales@surrey.ac.uk

Abstract

AIMS:

Circulating endogenous, dietary, and foreign chemicals can contribute to vascular dysfunction. The mechanism by which the vasculature protects itself from these chemicals is unknown. This study investigates whether the pregnane X receptor (PXR), the major transcriptional regulator of hepatic drug metabolism and transport that responds to such xenobiotics, mediates vascular protection by co-ordinating a defence gene programme in the vasculature.

METHODS AND RESULTS:

PXR was detected in primary human and rat aortic endothelial and smooth muscle cells (SMC) and blood vessels including the human and rat aorta. Metabolic PXR target genes cytochrome P450 3A, 2B, 2C, and glutathione S-transferase mRNA and activity were induced by PXR ligands in rodent and human vascular cells and absent in the aortas from PXR-null mice stimulated in vivo or in rat aortic SMC expressing dominant-negative PXR. Activation of aortic PXR by classical agonists had several protective effects: increased xenobiotic metabolism demonstrated by bioactivation of the pro-drug clopidogrel, which reduced adenosine diphosphate-induced platelet aggregation; increased expression of multidrug resistance protein 1, mediating chemical efflux from the vasculature; and protection from reactive oxygen species-mediated cell death.

CONCLUSION:

PXR co-ordinately up-regulates drug metabolism, transport, and antioxidant genes to protect the vasculature from endogenous and exogenous insults, thus representing a novel gatekeeper for vascular defence.

PMID:
22166712
PMCID:
PMC3291088
DOI:
10.1093/cvr/cvr330
[Indexed for MEDLINE]
Free PMC Article
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