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Plant Methods. 2011 Dec 13;7(1):45. doi: 10.1186/1746-4811-7-45.

An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants.

Author information

1
School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.
2
Syngenta, Jealotts Hill International Research Centre, Bracknell, Berkshire, UK.
3
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Dublin 4, Ireland.
4
Faculty of Life Science, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK.
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Contributed equally

Abstract

In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model described here is transferable between species, can be used to investigate rates of AL-PCD in response to various treatments and to identify modulation of AL-PCD rates in mutant/transgenic plant lines facilitating rapid screening of mutant populations in order to identify genes involved in AL-PCD regulation.

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