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Sensors (Basel). 2010;10(10):9286-316. doi: 10.3390/s101009286. Epub 2010 Oct 15.

Optical oxygen sensors for applications in microfluidic cell culture.

Author information

1
Department of Electrical & Computer Engineering, University of British Columbia/2332 Main Mall, Vancouver, BC V6T 1Z4, Canada. sgrist@ece.ubc.ca

Abstract

The presence and concentration of oxygen in biological systems has a large impact on the behavior and viability of many types of cells, including the differentiation of stem cells or the growth of tumor cells. As a result, the integration of oxygen sensors within cell culture environments presents a powerful tool for quantifying the effects of oxygen concentrations on cell behavior, cell viability, and drug effectiveness. Because microfluidic cell culture environments are a promising alternative to traditional cell culture platforms, there is recent interest in integrating oxygen-sensing mechanisms with microfluidics for cell culture applications. Optical, luminescence-based oxygen sensors, in particular, show great promise in their ability to be integrated with microfluidics and cell culture systems. These sensors can be highly sensitive and do not consume oxygen or generate toxic byproducts in their sensing process. This paper presents a review of previously proposed optical oxygen sensor types, materials and formats most applicable to microfluidic cell culture, and analyzes their suitability for this and other in vitro applications.

KEYWORDS:

cell culture; lab-on-a-chip; luminescence; microfluidics; optical oxygen sensors

PMID:
22163408
PMCID:
PMC3230974
DOI:
10.3390/s101009286
[Indexed for MEDLINE]
Free PMC Article

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