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Protein Sci. 2012 Feb;21(2):279-88. doi: 10.1002/pro.2014. Epub 2012 Jan 4.

Cell-free synthesis of membrane subunits of ATP synthase in phospholipid bicelles: NMR shows subunit a fold similar to the protein in the cell membrane.

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1
Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada.

Abstract

NMR structure determination of large membrane proteins is hampered by broad spectral lines, overlap, and ambiguity of signal assignment. Chemical shift and NOE assignment can be facilitated by amino acid selective isotope labeling in cell-free protein synthesis system. However, many biological detergents are incompatible with the cell-free synthesis, and membrane proteins often have to be synthesized in an insoluble form. We report cell-free synthesis of subunits a and c of the proton channel of Escherichia coli ATP synthase in a soluble form in a mixture of phosphatidylcholine derivatives. In comparison, subunit a was purified from the cell-free system and from the bacterial cell membranes. NMR spectra of both preparations were similar, indicating that our procedure for cell-free synthesis produces protein structurally similar to that prepared from the cell membranes.

PMID:
22162071
PMCID:
PMC3324772
DOI:
10.1002/pro.2014
[Indexed for MEDLINE]
Free PMC Article
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