Format

Send to

Choose Destination
Curr Protoc Cell Biol. 2011 Dec;Chapter 17:Unit 17.3.. doi: 10.1002/0471143030.cb1703s53.

Interaction trap/two-hybrid system to identify interacting proteins.

Author information

1
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.

Abstract

The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.

PMID:
22161546
DOI:
10.1002/0471143030.cb1703s53
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center