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Oncogene. 2012 Aug 16;31(33):3777-84. doi: 10.1038/onc.2011.564. Epub 2011 Dec 12.

The 14q22.2 colorectal cancer variant rs4444235 shows cis-acting regulation of BMP4.

Author information

1
Section of Cancer Genetics, Institute of Cancer Research, Surrey, UK.

Abstract

Common genetic variation at human 14q22.2 tagged by rs4444235 is significantly associated with colorectal cancer (CRC) risk. Re-sequencing was used to comprehensively annotate the 17kb region of strong linkage disequilibrium encompassing rs4444235. Through bioinformatic analyses using H3K4Me1, H3K4Me3, and DNase-I hypersensitivity chromatin signatures and evolutionary conservation we identified seven candidate disease-causing single-nucleotide polymorphisms mapping to six regions within the 17-kb region predicted to have regulatory potential. Reporter gene studies of these regions demonstrated that the element to which rs4444235 maps acts as an allele-specific transcriptional enhancer. Allele-specific expression studies in CRC cell lines heterozygous for rs4444235 showed significantly increased expression of bone morphogenetic protein-4 (BMP4) associated with the risk allele (P<0.001). These data provide evidence for a functional basis for the non-coding risk variant rs4444235 at 14q22.2 and emphasizes the importance of genetic variation in the BMP pathway genes as determinants of CRC risk.

PMID:
22158048
DOI:
10.1038/onc.2011.564
[Indexed for MEDLINE]

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