Format

Send to

Choose Destination
EMBO J. 2012 Feb 15;31(4):986-99. doi: 10.1038/emboj.2011.450. Epub 2011 Dec 13.

Dynamic long-range chromatin interactions control Myb proto-oncogene transcription during erythroid development.

Author information

1
Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands.

Abstract

The key haematopoietic regulator Myb is essential for coordinating proliferation and differentiation. ChIP-Sequencing and Chromosome Conformation Capture (3C)-Sequencing were used to characterize the structural and protein-binding dynamics of the Myb locus during erythroid differentiation. In proliferating cells expressing Myb, enhancers within the Myb-Hbs1l intergenic region were shown to form an active chromatin hub (ACH) containing the Myb promoter and first intron. This first intron was found to harbour the transition site from transcription initiation to elongation, which takes place around a conserved CTCF site. Upon erythroid differentiation, Myb expression is downregulated and the ACH destabilized. We propose a model for Myb activation by distal enhancers dynamically bound by KLF1 and the GATA1/TAL1/LDB1 complex, which primarily function as a transcription elongation element through chromatin looping.

PMID:
22157820
PMCID:
PMC3280550
DOI:
10.1038/emboj.2011.450
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center