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Eye Contact Lens. 2012 Jan;38(1):27-35. doi: 10.1097/ICL.0b013e31823f7041.

Dose-dependent and synergistic effects of proteoglycan 4 on boundary lubrication at a human cornea-polydimethylsiloxane biointerface.

Author information

1
Faculty of Kinesiology, Human Performance Laboratory, University of Calgary, Calgary, Canada.

Abstract

OBJECTIVES:

Proteoglycan 4 (PRG4), also known as lubricin, is a boundary lubricating mucin-like glycoprotein present on several tissue surfaces in the body. The objectives of this study were to (1) implement and characterize an in vitro boundary lubrication test at a human cornea-polydimethylsiloxane (PDMS) biointerface and (2) determine the dose-dependent and synergistic effects of PRG4, with hyaluronan (HA), on ocular surface boundary lubrication using this test.

METHODS:

Human corneas and model PDMS material were articulated against each other, at effective sliding velocities v(eff) between 0.3 and 30 mm/sec under physiologic loads of approximately 8 to 25 kPa. Samples were tested serially in (1) saline, PRG4 at 30, 100, 300 μg/mL resuspended in saline, then saline again or (2) saline, AQuify Comfort Eye Drops (containing 0.1% HA), 300 μg/mL PRG4 in saline, 300 μg/mL PRG4 in AQuify, then saline again. Both static and kinetic friction coefficients were calculated.

RESULTS:

PRG4 effectively lowered friction at the cornea-PDMS biointerface, both alone in a dose-dependent manner and in combination with HA. PRG4 reduced kinetic friction coefficients, <μ(kinetic, Neq)>, from approximately 0.30 in saline, to approximately 0.30, 0.24, and 0.17 in 30, 100, and 300 μg/mL PRG4, respectively. Values of <μ(kinetic, Neq)> in AQuify, approximately 0.32, were similar to those in saline; however, when combined with 300 μg/mL PRG4, values of <μ(kinetic, Neq)> were reduced to approximately 0.15.

CONCLUSIONS:

PRG4 functions as an effective ocular surface boundary lubricant, both alone in a dose-dependent manner and in combination with HA.

PMID:
22157393
DOI:
10.1097/ICL.0b013e31823f7041
[Indexed for MEDLINE]

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