Format

Send to

Choose Destination
See comment in PubMed Commons below
Biochem Biophys Res Commun. 2012 Jan 6;417(1):280-6. doi: 10.1016/j.bbrc.2011.11.101. Epub 2011 Nov 29.

The role of Roquin overexpression in the modulation of signaling during in vitro and ex vivo T-cell activation.

Author information

1
School of Life Sciences and Biotechnology, Kyungpook National University, Buk-ku, Daegu, Republic of Korea.

Abstract

The T-cell receptor (TCR) engages with an antigen and initiates a signaling cascade that leads to the activation of transcription factors. Roquin, a protein encoded by the RC3H1 gene and characterized as an immune regulator, was recently identified as a novel RING-type ubiquitin ligase family member, but the mechanisms by which Roquin regulates T-cell responses are unclear. We used the EL-4 murine lymphoma cell line to elucidate the role of Roquin in vitro. Roquin-overexpressing EL-4 cells became hyper-responsive after anti-CD3/CD28 stimulation in vitro and were a major source of the cytokines IL-2 and TNF-α. Upon activation, these cells showed particularly enhanced production of IL-2 and TNF-α. To clarify the important role played by Roquin in T-cell responses ex vivo, we generated T-cell-specific Roquin transgenic (Tg) mice. Roquin-Tg CD4(+) T-cells showed enhanced production of IL-2 and TNF-α in response to TCR stimulation with anti-CD28 co-stimulation. Further studies are necessary to investigate the role of Roquin in the regulation of primary T-cell activation, survival, and differentiation.

PMID:
22155229
DOI:
10.1016/j.bbrc.2011.11.101
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center