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Trends Biochem Sci. 2012 Feb;37(2):49-57. doi: 10.1016/j.tibs.2011.10.005. Epub 2011 Dec 7.

MultiBac: expanding the research toolbox for multiprotein complexes.

Author information

1
European Molecular Biology Laboratory (EMBL), Grenoble Outstation, UJF-CNRS-EMBL Unite Mixte International UMI 3265, rue Jules Horowitz, 38042 Grenoble Cedex 9, France.

Abstract

Protein complexes composed of many subunits carry out most essential processes in cells and, therefore, have become the focus of intense research. However, deciphering the structure and function of these multiprotein assemblies imposes the challenging task of producing them in sufficient quality and quantity. To overcome this bottleneck, powerful recombinant expression technologies are being developed. In this review, we describe the use of one of these technologies, MultiBac, a baculovirus expression vector system that is particularly tailored for the production of eukaryotic multiprotein complexes. Among other applications, MultiBac has been used to produce many important proteins and their complexes for their structural characterization, revealing fundamental cellular mechanisms.

PMID:
22154230
DOI:
10.1016/j.tibs.2011.10.005
[Indexed for MEDLINE]

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