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Anal Chim Acta. 2012 Jan 20;711:7-16. doi: 10.1016/j.aca.2011.09.042. Epub 2011 Nov 4.

Liquid chromatography-mass spectrometry based global metabolite profiling: a review.

Author information

1
Department of Chemistry, Aristotle University, Thessaloniki, Greece. gtheodor@chem.auth.gr

Abstract

Untargeted, global metabolite profiling (often described as metabonomics or metabolomics) represents an expanding research topic and is, potentially, a major pillar for systems biology studies. To obtain holistic metabolic profiles from complex samples, such as biological fluids or tissue extracts, requires powerful, high resolution and information-rich analytical methods and for this spectroscopic technologies are generally used. Mass spectrometry, coupled to liquid chromatography (LC-MS), is increasingly being used for such investigations as a result of the significant advances in both technologies over the past decade. Here we try to critically review the topic of LC-MS-based global metabolic profiling and describe and compare the results offered by different analytical strategies and technologies. This review highlights the current challenges, limitations and opportunities of the current methodology.

PMID:
22152789
DOI:
10.1016/j.aca.2011.09.042
[Indexed for MEDLINE]

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