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Anal Chim Acta. 2012 Jan 20;711:7-16. doi: 10.1016/j.aca.2011.09.042. Epub 2011 Nov 4.

Liquid chromatography-mass spectrometry based global metabolite profiling: a review.

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Department of Chemistry, Aristotle University, Thessaloniki, Greece.


Untargeted, global metabolite profiling (often described as metabonomics or metabolomics) represents an expanding research topic and is, potentially, a major pillar for systems biology studies. To obtain holistic metabolic profiles from complex samples, such as biological fluids or tissue extracts, requires powerful, high resolution and information-rich analytical methods and for this spectroscopic technologies are generally used. Mass spectrometry, coupled to liquid chromatography (LC-MS), is increasingly being used for such investigations as a result of the significant advances in both technologies over the past decade. Here we try to critically review the topic of LC-MS-based global metabolic profiling and describe and compare the results offered by different analytical strategies and technologies. This review highlights the current challenges, limitations and opportunities of the current methodology.

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