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J Am Chem Soc. 2012 Jan 11;134(1):103-6. doi: 10.1021/ja2090367. Epub 2011 Dec 14.

Structure of the stapled p53 peptide bound to Mdm2.

Author information

1
Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.

Abstract

Mdm2 is a major negative regulator of the tumor suppressor p53 protein, a protein that plays a crucial role in maintaining genome integrity. Inactivation of p53 is the most prevalent defect in human cancers. Inhibitors of the Mdm2-p53 interaction that restore the functional p53 constitute potential nongenotoxic anticancer agents with a novel mode of action. We present here a 2.0 Å resolution structure of the Mdm2 protein with a bound stapled p53 peptide. Such peptides, which are conformationally and proteolytically stabilized with all-hydrocarbon staples, are an emerging class of biologics that are capable of disrupting protein-protein interactions and thus have broad therapeutic potential. The structure represents the first crystal structure of an i, i + 7 stapled peptide bound to its target and reveals that rather than acting solely as a passive conformational brace, a staple can intimately interact with the surface of a protein and augment the binding interface.

PMID:
22148351
DOI:
10.1021/ja2090367
[Indexed for MEDLINE]

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