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World J Gastroenterol. 2011 Nov 21;17(43):4810-6. doi: 10.3748/wjg.v17.i43.4810.

Schistosoma japonicum ova maintains epithelial barrier function during experimental colitis.

Author information

1
Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Abstract

AIM:

To evaluate the impacts of Schistosoma japonicum (S. japonicum) ova on the tight junction barriers in a trinitrobenzenesulfonic acid (TNBS)-induced colitis model.

METHODS:

Balb/c mice were randomly divided into three groups: control group; TNBS(+)ova(-) group and TNBS(+)ova(+) group. TNBS was used intracolonic to induce colitis and mice of the TNBS(+)ova(+) group were pre-exposed to S. japonicum ova as a prophylactic intervention. Colon inflammation was quantified using following variables: mouse mortality, weight loss, colon extent and microscopic inflammation score. Serum expression of tumor necrosis factor-α and interferon-γ were assessed to evaluate the systemic inflammatory response. NOD2 and its mRNA were also tested. Bacterial translocations were tested by culturing blood and several tissues. ZO-1 and occludin were chosen as the representations of tight junction proteins. Both the proteins and mRNA were assessed.

RESULTS:

Ova pre-treatment contributed to the relief of colitis and decreased the mortality of the models. NOD2 expression was significantly downregulated when pretreated with the ova. The TNBS injection caused a significant downregulation of ZO-1 and occludin mRNA together with their proteins in the colon; ova pre-exposure reversed these alterations. Treatment with S. japonicum ova in the colitis model caused lower intestinal bacterial translocation frequency.

CONCLUSION:

S. japonicum ova can maintain epithelial barrier function through increasing tight junction proteins, thus causing less exposure of NOD2 to the luminal antigens which may activate a series of inflammatory factors and induce colitis.

KEYWORDS:

Crohn’s disease; Occludin; Schistosoma japonicum ova; Tight junction protein; ZO-1

PMID:
22147983
PMCID:
PMC3229631
DOI:
10.3748/wjg.v17.i43.4810
[Indexed for MEDLINE]
Free PMC Article

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