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J Enzyme Inhib Med Chem. 2012 Dec;27(6):880-5. doi: 10.3109/14756366.2011.637202. Epub 2011 Dec 6.

Simple methanesulfonates are hydrolyzed by the sulfatase carbonic anhydrase activity.

Author information

1
Department of Chemistry, Art and Science Faculty, Ağri Ibrahim Çeçen University, Ağri, Turkey.

Abstract

The possible sulfatase activity of several carbonic anhydrase (CA, EC 4.2.1.1) isoforms have been investigated with a series of synthesized methanesulfonate derivatives of phenols. Four α-CA isozymes, i.e. hCA I, hCA II, hCA IV and hCA VI (h = human isoform), were included in the study. We evidenced that the original sulfonate esters are being hydrolyzed effectively to the corresponding phenols which there after act as CA inhibitors. The K(I)-s of these compounds ranged from 10.24 to 4012 µM against hCA I, 0.10 to 35.42 µM against hCA II, 0.49 to 45.06 µM against hCA IV and 3.27 to 608 µM against CA VI, respectively. The relevant sulfatase activity of CA with these esters is amazing considering the fact that 4-nitrophenyl-sulfate, an activated ester, is not a substrate of these enzymes.

PMID:
22145694
DOI:
10.3109/14756366.2011.637202
[Indexed for MEDLINE]

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