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Stem Cells Dev. 2012 Aug 10;21(12):2239-51. doi: 10.1089/scd.2011.0607. Epub 2012 Jan 26.

Helios transcription factor expression depends on Gsx2 and Dlx1&2 function in developing striatal matrix neurons.

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1
Department of Cell Biology, Immunology and Neuroscience, and Cell Therapy Program, Faculty of Medicine, Institut d'Investigacions Biomèdiques August Pi i Sunyer-IDIBAPS, University of Barcelona, Barcelona, Spain.

Abstract

Development of the nervous system is finely regulated by consecutive expression of cell-specific transcription factors. Here we show that Helios, a member of the Ikaros transcription factor family, is expressed in ectodermal and neuroectodermal-derived tissues. During embryonic development, Helios is expressed by several brain structures including the lateral ganglionic eminence (LGE, the striatal anlage); the cingulated, insular and retrosplenial cortex; the hippocampus; and the accessory olfactory bulb. Moreover, Helios is also expressed by Purkinje neurons during postnatal cerebellar development. Within the LGE, Helios expression follows a dynamic spatio-temporal pattern starting at embryonic stages (E14.5), peaking at E18.5, and completely disappearing during postnatal development. Helios is expressed by a small population of nestin-positive neural progenitor cells located in the subventricular zone as well as by a larger population of immature neurons distributed throughout the mantle zone. In the later, Helios is preferentially expressed in the matrix compartment, where it colocalizes with Bcl11b and Foxp1, well-known markers of striatal projection neurons. In addition, we observed that Helios expression is not detected in Dlx1/2 and Gsx2 null mutants, while its expression is maintained in Ascl1 mutants. These findings allow us to introduce a new transcription factor in the cascade of events that take part of striatal development postulating the existence of at least 4 different neural progenitors in the LGE. An Ascl1-independent but Gsx2- & Dlx1/2-dependent precursor will express Helios defining a new lineage for a subset of matrix striatal neurons.

PMID:
22142223
PMCID:
PMC3411368
DOI:
10.1089/scd.2011.0607
[Indexed for MEDLINE]
Free PMC Article
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